PROTEIN
TOOLS
Prestained
Protein Marker, Low Range, Dual colors
|
Description
FAVORGEN® Protein Markers , low range, resolved to 6 sharp bands when analyzed by SDS-PAGE (Tris-Glycine), are mixture of purified proteins with known amino acid sequences. They are provided in a loading buffer containing SDS, reductant and glycerol for SDS-PAGE. Protein Markers are available for SDS-PAGE, IEF, 2-D, and Western blotting.
Applications
★ Molecular weight estimations
★ Assessing transfer efficiency
★ Monitoring electrophoretic separation
Information
★ Sharpest unstained bands
★ Best for molecular weight estimations
★ Load-and-to format; no dilution
Storage Condition
★ 70mM Tris-HCl/ 5% 2-mercaptoethanol/ 33mM NaCl /40mM dithiothreitol/ 1mM EDTA/ 10% glycerol/ 2% SDS (w/v) pH 6.8 at 25 ℃
★ Aliquot and prevent freeze and thaw cycles
★ Shelf Life: one year at -20 ℃
Apparent Molecular Weight of Protein Markers |
Protein |
Source |
Molecular Weight (Daltons) |
Phosphorylase b |
Rabbit Muscle |
100,000 |
Serum albumin |
Bovine |
76,000 |
Ovalbumin |
Egg White |
54,000 |
Carbonic Anhydrase |
Bovine Erythrocytes |
31,000 |
Trypsin Inhibitor |
Soybean |
24,000 |
α-Lactalbumin |
Bovine Milk |
14,000 |
Effective Size Range : 14kDa to 100kDa
Concentration: Approximately 2.6mg/ml
Usage Note
★ Bring desired amount of the Prestained protein marker over to a separate tube. For blotting: use 5 μl for mini-gels and 10μl for full length gels. For visualizing during electrophoresis: use 5~10μl for mini-gels and 10~20μl for full length gels.
★ Heat the Marker to 37-40℃ for 1 minutes if any solids was precipitated at -20℃.
★ After a quick microcentrifuge spin, load directly on to a gel.
Ordering Information |
Cat. No. |
Product Name |
Conc. |
Package |
Quantity |
Size Range |
No. of Bands |
FAPM-001-1 |
Prestained Protein Marker, Low Range, Dual Colors-Blue/Red |
2.6mg/m
|
500μl /vial |
100 lanes |
14, 24, 31, 54, 76, 100kDa |
6 |
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