PROTEIN
TOOLS
Protein assay Kit
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Description
The Favorgen protein assay kit, based on the Bradford dye-binding method ( Bradford 1976), is a simple colorimetric assay for measuring concentration of solubilized protein. The kit depends on an acidic dye, binding with protein measured at 595nm by a spectrophotometer or microplate reader. Comparison to a standard curve provides quantitation of unknown protein concentration.
Applications
¡¹ Reagents Compatible
¡¹ Quantitates Most Proteins
¡¹ Easy to adapt the assay from the standard-concentration range to a low-concentration
Additional Mateiral Required
• Spectrophotometer or microplate reader set to 595nm
• Cuvettes or microplate
Method and linear range
I. Standard procedure
II. Microassay procedure
linear and working range
Method I.
linear range (BSA) ¡G 100 £g g/ml - 1000 £g g/ml
Method II.
linear range (BSA)¡G1£gg/ml - 20£gg/ml
Method III.
linear range (BSA)¡G40£gg/ml - 500£gg/ml
Method IV.
linear range (BSA)¡G1£gg/ml - 20£gg/mll
Compare With B brand by Method II
Usage Note
¡¹ If your sample protein concentration is relative higher concentration between 100 £gg/ml and 1 mg/ml, then the Method 1 is suggested.
¡¹ If your sample protein concentration is between 1 £gg/ml and 20 £gg/ml, then the Method 2 or 4 can be used.
¡¹ If your sample protein concentration is between 40£gg/ml-500£gg/ml, then Method 3 is suggested.
Ordering Information |
Cat. No. |
Product Name |
Q'ty |
Package |
FAPAK-001 |
Favorgen Protein Assay Kit |
150ml |
1. 150ml dye reagent concentrate
2. 0.5ml 10mg/ml BSA |
FAPAK-002 |
Favorgen Protein Assay Kit |
450ml |
1. 450ml dye reagent concentrate
2. 0.5ml 10mg/ml BSA |
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