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NUCLEIC ACIDS EXTRACTION

Tissue Total RNA
Mini / Midi / Maxi Kit

 

 

 

 

 

Description
The Tissue Total RNA Purification Mini Kit is designed for purification of total RNA from tissue using the chaotropic salt-lysis method without the use of hazardous solvents such as phenol. The Kit can quickly purify total RNA from up to 10mg of tissues within 30 minutes. The purified RNA is suitable for direct use in RT-PCR, Northern blotting, primer extension and cDNA library construction.

Features
•  High Purity: OD260/280: 1.9-2.1
•  High Speed: Rapid speed for the purification high quality RNA from samples ranging in size from 10mg of tissues in about 20~30 minutes, depending on the amount of starting material.
•  SafeUse: Eliminates the use of phenol, chloroform, ethidium bromide, and cesium chloride, minimizing exposure to, and disposal of hazardous materials.

Time Required
 About 20~30 minutes .

Applications
•  Nothern blotting hybridizations
•  Primer extension
•  RT-PCR
•  RNase protection assays
•  Differential display
•  As starting material for purification of mRNA for cDNA synthesis

Sample Size
Up to 20mg Tissue

Yield of Purification

Sample
Size
RNA Yield(£gg)
Liver
10 mg
20~30
Brain
10 mg
5~10
Lung
10 mg
5~10
Heart
10 mg
5~10
Kidney
10 mg
20~30
Bacterial
1x109
30~50

Agarose gel of total RNA purified with the tissue Total RNA mini kit

5 £gl of elution product from 10mg of different tissues was loaded to each lane.
All tissues were from a mouse.
1. Brain
2. Lung
3. Liver
4. Spleen
5. Kidney

Procedure

The method uses detergents and a chaotropic salt to lysis cell and inactivate RNase, then RNA in chaotropic salt is bonded to the glass fiber matrix of column. After washing off the contaminants, the purified RNA is eluted by RNase-free water. The entire procedure can be completed about 20~30 minutes.

Storage Conditions
FavorPrep™ Tissue Total RNA Mini Kit can be stored at room temperature (15-25 ¢J ). Stable for 1 year at room temperature at 15-25¢J.

Ordering Information
Cat. No. Product Name Size Kit Contents Store at

FATRK 001

 

FavorPrep™ Tissue Total RNA Mini Kit

50 preps.

FARB Buffer
Wash1 Buffer 1
Wash Buffer 2 (conc.)
RNase-Free Water
FARB Mini Columns
2 ml collection tubes
Micropestles (RNase Free)

Store at room temperature at 15-25 ¢J for 1 year.

FATRK 001-1

100 preps

FATRK 001-2

300 preps

FATRK 002

 

FavorPrep™ Tissue Total RNA Midi Kit

20 preps.

FARB Buffer
Wash1 Buffer 1
Wash Buffer 2 (conc.)
RNase-Free Water
FARB Midi Columns

Store at room temperature at 15-25 ¢J for 1 year.

FATRK 002-1

50 preps

FATRK 003

 

FavorPrep™ Tissue Total RNA Maxi Kit

10 preps.

FARB Buffer
Wash1 Buffer 1
Wash Buffer 2 (conc.)
RNase-Free Water
FARB Maxi Columns

Store at room temperature at 15-25 ¢J for 1 year.

FATRK 003-1

24 preps