NUCLEIC ACIDS EXTRACTION
FavorPrep™ Plasmid DNA Extraction Mini Kit
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Description
The FavorPrep™ Plasmid Extraction Kit provide a rapid, phenol-free method for the extraction of high-purity plasmid DNA from bacterial cultures such as E. coli, which bacteria is pelleted, lysed, and then neutralized. The extracted DNA can be used in a variety of applications such as PCR, cloning, sequencing, in vitro transcription, and labeling. Also, as a column-type tube is utilized in the purification process, extraction is carried out in three simple steps of binding/washing/elution. Once bound, the DNA is washed and then eluted from the column, ready for use.
Features
• For high yields of plasmid DNA-up to 30£gg from 1~5ml overnight cultures.
• Effective purification of DNA fragments ranging from 100bp to 12+kb.
• No need for messy resin slurries, extracting with phenol, or concentrating via alcohol precipitation.
• Superior purity-DNA yields quality sequence data using automated or manual methods.
• Optimized buffers are included for maximum DNA purity and yield.
• Versatile protocol-works with all neutral gel buffers and both conventional and low-melting agarose gel.
Time Required
Under 30 minutes
DNA Sequencing analysis
Apparatus: ABIPRISM 3730
Protocols: DYEnamic? ET terminator cycles sequencing ready
Reaction Kit: FavorPrep Plasmid DNA Extraction Kit
Template: pGEM-T
Applications
• Sequencing
• Ligation
• Restriction enzyme digestion
• Transformation
Agarose Gel Analysis Of Plasmids Purified
Restriction analysis of 4 plasmids extracted by FavorPrep Plasmid DNA
Extration Kit. Digestion with EcoRI.
M: 1kb DNA ladder (100bp-12,000bp)
1: pCAMBIA 1304 cloned with 1,600bp EoRI insert (13kb)
2. pBluescript (2.9kb)
3: pF1 (11kb)
4: pR1 (5.3kb)
Storage Conditions
FavorPrep™ Plasmid DNA Extraction Kit can be stored at room temperature (15-25 ¢J ). After adding RNase A, PDE Buffer1, should be stored at 4 ¢J and is stable for six months. Other buffers and columns can be stored dry for up to 1 year at room temperature (15-25 ¢J ).
| Ordering Information |
| Cat. No. |
Product Name |
Size |
Kit Contents |
Store at |
FAPDE 001 |
FavorPrep™ Plasmid DNA Extraction Kit |
100 preps. |
1. PDE Buffer 1 25ml
2. PDE Buffer 2 25ml
3. PED Buffer 3 45ml
4. Wash Buffer1 45ml
5. Wash Buffer 2 12.5ml
6. Elution Buffer 6ml
7. RNase (100 mg/ml) 50£gl
8. PDE Column 100 preps
9. Collection Tube 100 tubes |
PDE Buffer with RNaseA at 4 ¢J , others at room temperature. |
FAPDE 001-1 |
FavorPrep™ Plasmid DNA Extraction Kit |
300 preps. |
1. PDE Buffer 1 65ml
2. PDE Buffer 2 75ml
3. PED Buffer 3 100ml
4. Wash Buffer 1 130ml
5. Wash Buffer (conc.)50ml
6. Elution Buffer 30ml
7. RNase (100 mg/ml) 130£gl
8. PDE Column 300 preps
9. Collection Tube 300 tubes
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PDE Buffer with RNaseA at 4 ¢J , others at room temperature. |
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