NUCLEIC ACIDS EXTRACTION

PCR
Clean-Up Kit

GEL Purification Kit

GEL/PCR Purification Kit

MicroElute GEL/PCR Purification Kit

Description
The FavorPrep™ PCR Clean-Up Kit is designed for cleanup DNA fragments from PCR and other enzymatic products. The size range for effective fortification is 100bp ~10kb, thus common 20 ~40 mer oligonucleotides are removed. The recovery yield exceeds 80%. Elution volume can be as little as 30μL when concentrated product is needed. The purified DNA can be used directly for any downstream application.

Features
• Quick and easy to use, just three simple steps.
• Complete removal of primers and primer dimmers.
• PCR product is eluted into sterile water or elution buffer (supplied).
• No phenol/chloroform extraction and ethanol precipitation required.

Applications
Purified DNA is ready for downstream application such as sequencing, ligation, labeling, amplification and enzymatic digestion.

Sample Source
PCR products, labeled, modified or digested DNA (Ex. Restriction digests, alkaline phosphatase fragments, kinase reaction, enzymatic labeling reactions)

DNA Size Range
100bp ~10kb

Binding Capacity of Spin Filter
10 ~100 μl of PCR product

Recovery Rate
80 ~95%

Handling Time
About 20 minutes

The Quality of DNA after Purification

Purification of DNA fragments from PCR product by the PCR Clean-Up Kit. Recovery is about 85%.

M: 1Kb DNA Ladder (100bo-12,000bp)
1: 325bp PCR product before purified
2: 325bp PCR product after purified
3: 666bp PCR product before purified
4: 666bp PCR product after purified

Procedure

Storage Conditions
Stable for 1 year at room temperature. The kit is shipped at ambient temperature.