NUCLEIC ACIDS EXTRACTION
Blood/Cultured Cell Total RNA
Mini / Midi / Maxi Kit
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Description
The Blood/Cultured Cell Total RNA Purification Mini Kit is designed for purification of total RNA from cultured cells and fresh whole blood using the chaotropic salt-lysis method without the use of hazardous solvents such as phenol. The Kit can quickly purify total RNA from up to 300£gl of blood within 30 minutes. The purified RNA is suitable for direct use in RT-PCR, Northern blotting, primer extension and cDNA library construction.
Features
• High Purity: OD260/280: 1.9-2.1
• High Speed: Rapid speed for the purification high quality RNA from samples ranging in size from 200 £gl of blood or from 106 of cells in about 30 minutes.
• SafeUse: Eliminates the use of phenol, chloroform, ethidium bromide, and cesium chloride, minimizing exposure to, and disposal of hazardous materials.
• Scalable protocol: Solution based procedure can be scaled to suit the application.
Time Required
About 30-60 minutes.
Applications
• Nothern blotting hybridizations
• Primer extension
• RT-PCR
• RNase protection assays
• Differential display
• As starting material for purification of mRNA for cDNA synthesis
Sample Source
Whole Blood/Buffy Coat; Cultured Animal Cells, Gram ¡Vve/+ve Bacteria
Sample Size
Up to 1x 107 animal cultured cells, 1x109 bacterial cells, or 300£gl blood.
Yield of Purification
Sample |
Size |
RNA Yield |
|
Bacterial Cells |
1x109 |
30~50 |
|
Cultured Cells |
1x106 |
5~20 |
|
Blood |
200£gl |
4~12 |
Procedure
The method uses detergents and a chaotropic salt to lysis cell and inactivate RNase, then RNA in chaotropic salt is bonded to the glass fiber matrix of column. After washing off the contaminants, the purified RNA is eluted by RNase-free water. The entire procedure can be completed about 20~30 minutes.
Storage Conditions
FavorPrep™ Blood/Cultured Cell Total RNA Mini Kit can be stored at room temperature (15-25 ¢J ). Stable for 1 year at room temperature at 15-25¢J.
| Ordering Information |
| Cat. No. |
Product Name |
Size |
Kit Contents |
Store at |
FABRK 001 |
FavorPrep™ Blood/Cultured Cell Total RNA Mini Kit |
50 preps. |
RL Buffer
FARB Buffer
Wash Buffer 1
Wash Buffer 2 (conc.)
RNase-Free Water
FARB Columns
2ml collection tubes
Filter Column
|
Store at room temperature at 15-25 ¢J for 1 year. |
FABRK 001-1 |
100 preps |
FABRK 001-2 |
300 preps |
FABRK 002 |
FavorPrep™ Blood/Cultured Cell Total RNA Midi Kit |
20 preps. |
RL Buffer
FARB Buffer
Wash Buffer 1
Wash Buffer 2 (conc.)
RNase-Free Water
FARB Columns
Collection tubes
Filter Column |
Store at room temperature at 15-25 ¢J for 1 year. |
FABRK 002-1 |
50 preps |
FABRK 003 |
FavorPrep™ Blood/Cultured Cell Total RNA Maxi Kit |
10 preps. |
RL Buffer
FARB Buffer
Wash Buffer 1
Wash Buffer 2 (conc.)
RNase-Free Water
FARB Columns
Collection tubes
Filter Column |
Store at room temperature at 15-25 ¢J for 1 year. |
FABRK 003-1 |
24 preps |
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