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NUCLEIC ACIDS EXTRACTION

Blood / Cultured Cell Genomic DNA Extraction Mini Kit

Blood / Cultured Cell Genomic DNA Extraction Midi / Maxi Kit

Description
The FavorPrep™ Blood Genomic DNA Extraction Kit is designed for rapid extraction of pure genomic DNA from whole blood, serum, buffy coat, body fluids, lymphocytes and cultured cell. As a column-type tube is utilized in the purification process in three simple steps of binding, washing and then elution for the safe and convenient extraction of high-purity genomic DNA.

Features
•  Extraction of genomic DNA from whole blood, plasma, serum, buffy coat, body fluids,
    lymphocytes and cultured cells.
•  Purified DNA is ready for downstream application such as PCR, Southern blotting.
•  Centrifugation-based method.
•  Efficiently remove cellular debair and inhibitors
•  No phenol/chloroform extraction and ethanol precipitation required.

Applications
PCR
Southern Blotting
Medicolegal Analysis

Sampling
Up to 200 £gl of whole blood (with anti-coagulant), plasma, serum, buffy coat, or body fluids; up to 5x105lymphocytes or cultured cells in 200£gl PBS.

Yield
About 5£gg of total DNA from 200£gl of human whole blood; up to 50£gg of total DNA, depends on the sample types and the number of cells in the sample.

Yield of Purification
Sample Amounts Yield

Whole Blood

200 £gl

3~6£gg

Whole Blood

1 ml

10~40£gg

Buffy Coat

200 £gl

30~60£gg

Cultured Cells

5*106

15 ~30£gg

Bacterial Cells

2*109

100~300£gg

The yield of genomic DNA is variable depending on the sample types.

Handling Time
About 60 minutes depending upon the sample types.

 

Storage Conditions
Stable for 1 year at room temperature. The Proteinase K Powder store at -20¢J.

Ordering Information
Cat. No. Product Name Size Kit Components Store at

FABGK 001

 

FavorPrep™ Blood / Cultured Cell Genomic DNA Extraction Mini Kit

 

50 preps.

Proteinase K Powder
FATL Buffer
FABG Buffer
W1 Buffer
Wash Buffer (conc.)
Elution Buffer
FABG Mini Columns
2ml Collection tubes
1.5ml Elution tubes

Store at room temperature for 1 year. (Except Proteinase K Powder, store at -2 0¢J.)

FABGK 001-1

100 preps.

FABGK 004

 

FavorPrep™ Blood / Cultured Cell Genomic DNA Extraction Mini Kit

 

100 preps.

RBC Lysis Buffer
FATL Buffer
FABG Buffer
W1 Buffer
Wash Buffer (conc.)
Elution Buffer
FABG Mini Columns
2ml Collection tubes

Store at room temperature for 1 year. (Except Proteinase K Powder, store at -2 0¢J.)

FABGK 004-1

1300 preps.