NUCLEIC ACIDS EXTRACTION

PCR Clean-Up Kit

GEL Purification Kit

GEL/PCR Purification Kit

MicroElute GEL/PCR Purification Kit

Description
The FavorPrep™ Gel/PCR Purification Kit is designed to recover or concentrate DNA Fragments (50bp-10Kb) from agarose gel, PCR or other enzymatic reaction. The unique dual purpose application and high yield DNA column make this kit exceptional value.

Features
• With simple steps, quick and easy to use. .
• Highly pure DNA (suitable for PCR).
• No phenol/chloroform extraction and ethanol precipitation required.

Applications
• PCR
• Fluorescent or Radioactive Sequencing
• Restriction Digestion
• DNA Labeling
• Ligation and Transformation

Sample Size
Up to 300mg agarose gel slice
Up to 100 μ l PCR product or other enzymatic reaction

DNA Size Range
100bp ~20kb

Binding Capacity of Spin Filter
20 ~100 μl of PCR product

Recovery Rate
80 ~90% for GEL extraction
90~95% for PCR Clean-Up

Handling Time
10 minutes for PCR clean up
20 minutes for GEL extraction

The Quality of DNA After Purification

DNA fragments before and after extraction with the GEL/PCR Purification Mini Kit
Lane 1, 3, 5, 7 before extraction: 200bp, 500bp, 2Kb, 3Kb.
Lane 2, 4, 6, 8 after extraction: 200bp, 500bp, 2Kb, 3Kb
M1: 100bp DNA Ladder
M2: 1Kb DNA Ladder

Procedure

The method uses a chaotropic salt, guanidine thiocyanante to dissolve the agarose gel and denature enzymes. The DNA fragment in the chaotropic salt is bond to the glass fiber matrix of the spin column. After washing off the contaminants, the purified DNA fragments are eluted by a low salt elution buffer or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from reaction mixture without phenol extraction and alcohol precipitation.

Storage Conditions
Stable for 1 year at room temperature. The kit is shipped at ambient temperature.