NUCLEIC ACIDS EXTRACTION
Blood / Cultured Cell Genomic DNA Extraction Midi / Maxi Kit |
Description
The Blood Cell Genomic DNA Extraction Maxi Kit is designed for purification of total DNA (including genomic, mitochondrial and viral DNA) from large volumes of blood sample or cultured cells using a safe protocol that eliminates the use of organic solvents such as phenol and chloroform. By using proteinase K and lysis buffer, the kits effectively lyse cells and degrade protein. Purified DNA with approximately 20-30 Kb of size is suitable for PCR or other enzymatic reactions.
Features
• High Purity: DNA is immediately suitable for a variety of applications, including amplification, digestion, PCR etc.
• High Speed: Rapid speed for the isolation of genomic DNA from blood, within 60 minutes.
• Easy Use: Based on a five-step process, purities genomic DNA without the use of caustic organic compounds.
• Safe Use : The kit uses a spin column tube and removes proteins, nucleases in cell. It is not necessary to treat the sample with harmful organic solvents such as phenol and chloroform.
Applications
PCR
Southern Blotting
Real time PCR
AFLP
RFLP
Sampling
Blood, other body fluids and cultured cells. Up to 10ml human whole blood
Yield
About 50£gg - 150£gg of total DNA from 5ml of human whole blood; up to 100-300£gg of total DNA from 10ml of human whole blood, depends on the sample types and the number of cells in the sample.
| Yield of Purification |
| Sample |
Amounts |
Yield |
Whole Blood |
5 ml |
50 ~150£gg |
Whole Blood |
10 ml |
100 ~300£gg |
The yield of genomic DNA is variable depending on the sample types. |
Handling Time
About 60 minutes depending upon the sample types.
Storage Conditions
Stable for 1 year at room temperature. The Proteinase K Powder store at -20¢J.
| Ordering Information |
| Cat. No. |
Product Name |
Size |
Kit Components |
Store at |
FABGK 002 |
FavorPrep™ Blood / Cultured Cell Genomic DNA Extraction Midi Kit |
520 preps. |
Proteinase K Powder
FABG Buffer
W1 Buffer
Wash Buffer (conc.)
Elution Buffer
FABG Midi Columns
15 ml Elution tubes |
Store at room temperature for 1 year. (Except Proteinase K Powder, store at -2 0¢J.) |
FABGK 002-1 |
50 preps. |
FABGK 002-3 |
FavorPrep™ Blood / Cultured Cell Genomic DNA Extraction Midi Kit
|
20 preps. |
Proteinase K Powder
FABG Buffer
FATX Buffer
W1 Buffer
Wash Buffer (conc.)
Elution Buffer
FABG Midi Columns
15 ml Elution tubes
|
Store at room temperature for 1 year. (Except Proteinase K Powder, store at -2 0¢J.) |
FABGK 002-4 |
50 preps. |
FABGK 003 |
FavorPrep™ Blood / Cultured Cell Genomic DNA Extraction Maxi Kit
|
10 preps. |
Proteinase K Powder
FABG Buffer
W1 Buffer
Wash Buffer (conc.)
Elution Buffer
FABG Maxi Columns
50 ml Elution tubes |
Store at room temperature for 1 year. (Except Proteinase K Powder, store at -2 0¢J.) |
FABGK 003-1 |
24 preps. |
FABGK 003-3 |
FavorPrep™ Blood / Cultured Cell Genomic DNA Extraction Maxi Kit
|
10 preps. |
Proteinase K Powder
FABG Buffer
FATX Buffer
W1 Buffer
Wash Buffer (conc.)
Elution Buffer
FABG Maxi Columns
50 ml Elution tubes
|
Store at room temperature for 1 year. (Except Proteinase K Powder, store at -2 0¢J.) |
FABGK 003-4 |
24 preps. |
|
