NUCLEIC ACIDS EXTRACTION

Nucarrier

Tri-RNA Reagent

After Tri-Reagent RNA Clean-Up Kit

miRNA Isolation Kit

RNA Stabilization Solution

Description
The FavorPrep After Tri-Reagent RNA Clean-Up Kit is designed for fast clean up RNA that be isolated by different methods, such as guanidine isothiocyanate/phenol chloroform extraction or lithium chloride / phenol chloroform extraction and is also suitable for fast clean up RNA from enzymatic reaction mixture, such as labeling or DNase digestion reactions. In the purification procedure, combining the efficient reagents with the convenient spin-column system, impurities will remove completely. The entire procedure is not required the phenol-chloroform extraction and can be finished within 10 minutes. After using this purification kit, the purified RNA is ready for RT-PCR and other downstream application.

Features
•  RNA clean up can be operated directly after the chloroform extraction without isopropanol precipitation.
•  Sample Size: Up to 100 μ l of RNA sample or enzymatic reaction mixture.
•  High purity: OD260/280: 1.9~21.
•  Binding Capacity: Up to 100g
•  Handling Time: Within 10 minutes
•  Expected Recovery: 85~95%
•  Format: Spin Column

Applications
•  Real-Time PCR
•  Northern blotting hybridization
•  Primer extension
•  Differential display
•  RNase protection assays
•  As starting material for the purification of mRNA for cDNA synthesis

Storage Conditions
Stable for 1 year at room temperature

Procedure