1 plate
FavorPrep™ 96-Well Total RNA Kit

Principle: Filter Plate (silica membrane)
Sample size: up to 1 x 10 animal cells or 50 mg tissues / preparation
Processing: centrifugation protocol or vacuum & centrifugation protocol
Operation time: < within 1 hr/ 96 preparation
RNA Binding capacity: up to 75 µg/ well
Elution volume: 50 ~ 75 µl

Important Notes:
1. Make sure everything is RNase-free when handling this kit.
2. Buffers provided in this system contain irritants. Wear gloves and lab coat when andling these buffers.
3. Caution: ß-mercaptoethanol is hazardous to human health. perform the procedures involving ß-M in a chemical fume hood.
4. Add RNase-free ethanol (96~100%) to Wash Buffer 1 and Wash Buffer 2 when first use.
5. Prepare RNase-free DNase 1 reaction buffer (1M NaCl, 10 mM MnCl2, 20 mM Tris-HCl, pH 7.0 at 25ºC) and make the final concentration of DNase I to 0.5 U/ μl.
6. Equipments required:
• For centrifugation protocol: A centrifuge is required, capable of 5,600 ~ 6,000 X g, with a swing -bucket rotor and the adaptor for 96-well plates.
• For vacuum protocol: A vacuun manifold for 96-well plate and a vaccum source reached to 15 inches Hg are required. (Alternative): If using centrifugation for Elution Step (STEP 9), a centrifuge equiment is required, capable of 5,600 ~ 6,000 X g, with a swing -bucket rotor and the adaptor for 96-well plate.


Additional Requirements:
1. ß-mercaptoethanol (ß-Me)
2. 96 ~100 % RNase - free ethanol
3. 70 % RNase - free ethanol