50 prep
FavorPrep™ Soil DNA Isolation Mini Kit (beads-beating lysis) (sample size: 0.5 g soil, sludge or sediment samples)

Sample: 0.25 ~ 0.5 g
Operation time: 60 min
Principle: spin column (silica membrane)
Elution volume: 50 ~ 200 µl

Important Notes:
1. Buffers provided in this system contain irritants. Wear gloves and lab coat when handling these buffers.
2. Check SDE1 Buffer before use, Warm SDE1 Buffer at 60°C for 10 minutes if any precipitate formd.
3. Add indicated volume of ethanol (96-100%) to Wash Buffer before use.
4. Prepare a heating block or a water bath to 70 °C. If DNA is isolated from gram positive bacteria, prepare a heating block or a water bath to 95 °C for another incubation.
5. All centrifuge steps are done at full speed (~18,000 x g) in a microcentrifuge.
6. Preheat Elution Buffer or ddH2O to 60°C for elution step.