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2 prep
FavorPrep™ Plasmid DNA Maxi Kit - Endotoxin Removal


Ordering Info:

NameFavorPrep™ Plasmid DNA Maxi Kit - Endotoxin Removal
Cat NO Cat NO: FAPDE005C-002

Related Product:





FAPDE005C-002(2 preps)
FAPDE005C-010(10 preps)
FAPDE005C-020(20 preps)
PDEA Buffer
14 ml
70 ml
140 ml
PDEB Buffer
14 ml
70 ml
140 ml
PDEC Buffer
14 ml
70 ml
140 ml
Endotoxin Remove Buffer
1.2 ml
6 ml
12 ml
W1 Buffer (concentrate)
7.5 ml
36 ml
72 ml
Wash Buffer (concentrate)
8 ml
40 ml
40 ml x 2
Elution Buffer
2 ml
25 ml
50 ml
RNase A
1.4 mg
7 mg
14 mg
Midi Binding Column
2 pcs
10 pcs
20 pcs
15 ml tubes
4 pcs
20 pcs
40 pcs

Principle: spin column (silica matrix)
Sample size: 30 ~ 60 ml cell culture
Operation time: < 60 minutes
Binding capacity: up to 450 µg/ column
Column applicability: centrifugation and vaccum

Important Notes:
1. Store RNase A at -20 °C upon recipit of kit.
2. Add 0.5 ml of PDEA Buffer to the RNase A tube, vortex the tube to dissolve the RNase A well. Transfer the total RNase A mixture back to the PDEA bottle. Mix well by vortexing and store the PDEA buffer at 4 °C.
3. Buffer provided in this kit contain irritants. Wear gloves and lab coat when handling these buffers.
4. Check PDEB Buffer before use to see if any precipitate formed, If yes, Warm Buffer PEDB in a 37 ºC waterbath to dissolve precipitates.
5. To avoid acidification of PDEB Buffer from CO2 in the air. Close the bottle immediately after use.
6. For centrifuge protocol, the centrifuge rotor for 50 ml tube should be swig-bucket type to make the plasmid bind symmetrically to the membranes.

Additional Requirements:
1. Pipettes and pipette tips
2. vortex
3. 100% ethanol
4. Swing bucket centrifuge for 50 ml centrifuge tube (4,500 ~ 6,000 x g) for centrifuge protocol
5. Vacuum manifold for vacuum protocol

Store at room temperature (15~ 25 ℃) for 2 year.
Except RNAse A Powder, store at -20℃.


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Headquarter

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No.37,Nong-Ke Rd., Ping-Tung 908, Taiwan
Tel: +886-8-762-1829
Fax: +886-8-762-0791
Email: technical@favorgen.com
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phone: +43 (0)1 292 82 80
fax: +43 (0)1 292 82 80-88
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