10 prep
FavorPrep™ Endotoxin-Free Plasmid Extraction Maxi Plus Kit.

Format/Principle: Anion-exchange chromatography (gravity-flow column)
Lysate clarification: Centrifugation
Sample Size: 60~120 ml of bacteria for high/low-copy number plasmids
Plasmid or constructs range: 3 kbp ~ 150 kbp
Column Binding Capacity: < 1.5 mg DNA/column

Important Notes:
1. Store RNase A at -20 °C upon recipit of kit.
2. Add 0.5 ml of PM1 Buffer to a RNase A tube, vortex the tube to mix well. Transfer the total RNase A mixture back to the PM1 bottle, mix well by vortexing and store the PM1 buffer at 4 °C.
3. If precipitates have formed in PM2 Buffer, warm the buffer in 37°C waterbath to dissolve preciptates.
4. Pre-chill PM3 Buffer at 4 °C before starting.

Additional Requirements:
1. 50 ml tubes
2. Refrigerated centrifuge capable of ≥ 5,000 x g and the centrifuge tube suitable for the centrifuge rotor
3. Isopropanol
4. 70% ethanol
5. TE buffer or ddH2O