2 prep
FavorPrep™ Endotoxin Free Plasmid DNA Extraction Maxi Kit (sample size: 120 ~ 240 ml culture cells; endotoxin removal buffer provided)

Technology: Anion-exchange chromatography (gravity-flow column)
Lysate clarification: Centrifugation
Sample Size: 120 ~ 240 ml of bacteria for high-copy number or low -copy number plasmid
Plasmid or constructs range: 3kbp ~ 150kbp
Binding Capacity: 1.5 mg / Maxi Column

Important Notes:
1. Store RNase A at -20 °C upon recipit of kit.
2. Add 0.5 ml of PM1 Buffer to a RNase A tube and vortex the tube to mix well. Transfer the total RNase A mixture back to the PM1 bottle and mix well by vortexing. Store the PM1 buffer at 4 °C.
3. If precipitates have formed in PM2 Buffer, warm the buffer in 37°C waterbath to dissolve preciptates.
4. Pre-chill PM3 Buffer at 4 °C before starting.

Additional Requirements:
1. 50 ml tubes
2. Refrigerated centrifuge capable of ≥ 5,000 x g and the centrifuge tube suitable for the centrifuge rotor
3. Isopropanol
4. 70% ethanol
5. TE buffer or ddH2O