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50 prep
FavorPrep™ Plasmid Midi SP Kit (sample size: 1~ 30 ml culture cells; filter syringe for lystae clearance)


Ordering Info:

NameFavorPrep™ Plasmid Midi SP Kit (sample size: 1~ 30 ml culture cells; filter syringe for lystae clearance)
Cat NO Cat NO: FAPDE 005-M-050

Related Product:





FAPDE005-M-050(50 preps)
FAPDE005-M-002(2 preps)
FAPDE005-M-025(25 preps)
PDEA Buffer
80ml
30ml
40ml
PDEB Buffer
80ml
30ml
40ml
PDEC Buffer
80ml
30ml
40ml
W1 Buffer (concentrate)
48ml
1.8ml
24ml
Wash Buffer (concentrate)
50ml
2.5ml
25ml
Elution Buffer
50ml
2ml
25ml
RNase A
8mg
0.3mg
4mg
Midi Binding Column
50pcs
2pcs
25pcs
5 ml Filter Syringe
50pcs
2pcs
25pcs
15 ml tubes
100pcs
4pcs
50pcs

Principle: spin column (silica matrix)
Sample size: up to 15 ml cell culture
Operation time: < 60 minutes
Binding capacity: up to 250 µg/ column
Column applicability: centrifugation and vaccum

Materials and equipmentsupplied by user:
1. Pipettes and pipette tips
2. vortex
3. 100% ethanol
4. Swing bucket centrifuge for 15 ml centrifuge tube (4,500 ~ 6,000 x g) for centrifuge protocol
5. Vacuum manifold for vacuum protocol

Important Notes:
1. Store RNase A at -20 °C upon recipit of kit.
2. Add 0.5 ml of PDEA Buffer to the RNase A tube, vortex the tube to dissolve the RNase A well. Transfer the total RNase A mixture back to the PDEA bottle. Mix well by vortexing and store the PDEA buffer at 4 °C.
3. Buffer provided in this kit contain irritants. Wear gloves and lab coat when handling these buffers.
4. Check PDEB Buffer before use to see if any precipitate formed, If yes, warm Buffer PEDB in a 37 ºC waterbath to dissolve precipitates.
5. To avoid acidification of PDEB Buffer from CO2 in the air. Close the bottle immediately after use.
6. For centrifuge protocol, the centrifuge rotor for 15 ml tube should be swig-bucket type to make the plasmid bind symmetrically to the membranes.

Store at room temperature (15~ 25 ℃) for 2 year.
Except RNAse A Powder, store at -20℃.


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Tel: +886-8-762-1829
Fax: +886-8-762-0791
Email: order@favorgen.com


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phone: +43 (0)1 292 82 80
fax: +43 (0)1 292 82 80-88
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