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25 prep
FavorPrep™ High Speed Plasmid Midi Kit (sample size: 60 ~ 120 ml culture cells; filter syringe for lysate clearance; plasmid binding disc substituting ethanol precipitation)


Ordering Info:

NameFavorPrep™ High Speed Plasmid Midi Kit (sample size: 60 ~ 120 ml culture cells; filter syringe for lysate clearance; plasmid binding disc substituting ethanol precipitation)
Cat NO Cat NO: FAPDE002-SP2

Related Product:





FAPDE 002-SP2(25 preps)
FAPDE 002-SP2-S(2 preps)
PEQ Buffer
135ml
12ml
PM1 Buffer
215ml
18ml
PM2 Buffer
215ml
18ml
PM3 Buffer
215ml
18ml
PW Buffer
270ml+60ml
27ml
PEL Buffer
215ml
18ml
RNase A
21.5mg
1.8mg
PM Midi Column
25pcs
2pcs
Filter Syringe
25pcs
2pcs
20 ml Syringe
25pcs
2pcs
3 ml Syringe
25pcs
2pcs
DNA Binding Disc
25pcs
2pcs

Technology: Anion-exchange chromatography (gravity-flow column)
Lysate clarification: Filtration
Sample Size/ preparation:
PM Mini Column (ion-exchange resin column):
up to 150 ml of bacteria culture for low-copy number plasmid
Binding Capacity: up to 800 µg of DNA
Appropriate plasmid size: 3kb ~ 300 kb
DNA Binding Disc (glass fiber disc):
Binding Capacity: up to 800 µg of DNA
Appropriate plasmid size: 2kp ~ 50 kb
Operation time: plasmid size ≤ 50 kb: 45 min
plasmid size > 50 kb: 90 min
Technology: Anion-exchange chromatography (gravity-flow column)
Lysate clarification: Filtration
Sample Size/ preparation:
PM Mini Column (ion-exchange resin column):
up to 150 ml of bacteria culture for low-copy number plasmid
Binding Capacity: up to 800 µg of DNA
Appropriate plasmid size: 3kb ~ 300 kb
DNA Binding Disc (glass fiber disc):
Binding Capacity: up to 800 µg of DNA
Appropriate plasmid size: 2kp ~ 50 kb
Operation time: plasmid size ≤ 50 kb: 45 min
plasmid size > 50 kb: 90 min

Additional requirement to be provided by user
1. Centrifuge rotor and bottle for up to 150 ml cell culture and capable of speed at 4,5000~6,000 x g
2. 50 ml centrifuge
3. Isopropanol
4. 70% ethanol
5. TE buffer, PH7.0 or ddH2O, PH7.0
Important Notes:
1. Store RNase A at -20 °C upon recipit of kit.
2. Add 0.5 ml of PM1 Buffer to a RNase A tube, vortex the tube to mix well. Transfer the total RNase A
mixture back to the PM1 bottle, mix well by vortexing and store the PM1 buffer at 4 °C.
3. If precipitates have formed in PM2 Buffer, warm the buffer in 37°C waterbath to dissolve preciptates.
4. Pre-chill PM3 Buffer at 4 °C before starting.

Store at room temperature (15~ 25 ℃) for 2 year.
Except RNAse A Powder, store at -20℃.


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Headquarter

PingTung Agricultural Biotechnology Park
No.37,Nong-Ke Rd., Ping-Tung 908, Taiwan
Tel: +886-8-762-1829
Fax: +886-8-762-0791
Email: technical@favorgen.com
Email: order@favorgen.com


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phone: +43 (0)1 292 82 80
fax: +43 (0)1 292 82 80-88
Email: r.englisch@favorgen-europe.com

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