2 prep
Plasmid DNA Extraction Midi Kit (sample size: 60~120 ml culture cells)

Technology: Anion-exchange chromatography (gravity-flow column)
Lysate clarification: centrifugation
Sample Size: 60 ~ 120 ml of bacteria for high-copy number or low -copy number plasmid
Plasmid or constructs range: 3kbp ~ 150kbp
Binding Capacity: 650 µg / Midi Column
Important Notes:
1. Store RNase A at -20 °C upon recipit of kit.
2. Adding the provided RNase A to PM1 Buffer:
add 1 ml of PM1 Buffer to a RNase A tube, vortex the tube to dissolve the RNase A completely. Transfer the total RNase A mixture back to the PM1 bottle, mix well by vortexing and store the PM1 buffer at 4 °C.
3. If precipitates have formed in PM2 Buffer, warm the buffer in 37°C waterbath to dissolve preciptates.
4. Pre-chill PM3 Buffer at 4 °C before starting.

Additional Requirements: 1. 50 ml tubes
2. Refrigerated centrifuge capable of ≥ 5,000 x g and the centrifuge tube suitable for the centrifuge rotor
3. Isopropanol
4. 70% ethanol
5. TE buffer or ddH2O